We analyzed several species of fish again (36 fishes) - Poecilia sp. (P.reiculata, P.wingei, wingei x reticulata hybrids, P.velifera) and Xiphophorus sp. (X.helleri, X. maculatus) as well as among themselves.
We found 3 isoforms of enzimes and, respectively with differences between Endler's guppy and all groups of Poecilia reticulata (2 wild populations, 2 breeds and few hybrids).
The difference from the Endler's guppy (P. wingei):
Endler's guppy / Colombia wild - the difference of 3 isoforms of enzymes (hereinafter I will write count of differences only)
Endler's guppy / Moscow-River population - 3
Moscow-river population / Columbia wild - 2
X. helleri green / X.maculatus (two breeds) - 7-8
X. maculatus yellow/ X.maculatus black - 3
P. velifera / X. helleri green - 6
P. veifera / X. maculatus (two breeds)- 7-8
P.velifera / Endler's guppy (or Moscow-river population or Columbia wild) - 7
X. helleri green / Moscow-river population (or Columbia wild) - 8
Moscow guppy / Non-Moscow guppy (Italian breeding) - 2
Poecilia sp. differed from each other by 1-7 enzymes.
But, between Endler's guppy, all breeds and populations of guppies difference was 1-3, and only with P. velifera it was 7.
Two wild populations of guppies (Columbia and Moscow populations) showed a difference between to 2 enzymes, and between Endler's guppy and each of these populations - the difference was 3 enzyme. In this case, the difference between Moscow guppies and Italian non-Moscow breed was 2 enzymes.
Xiphophorus sp. differed from each other by 4-8 enzymes.
At the same time for comparison Molly differed from all the guppies and Endler and 7 enzymes.
Thus, representatives of the Poecilia sp. and Xiphophorus sp. differed among themselves (intergeneric differences) 6-8 enzymes.